正相 Silica 層析管柱 Cyano 層析管柱 逆相 C8 層析管柱 C18 層析管柱 蛋白質分析 毛細管層析管柱 C8 Wide Pore 層析管柱 C18 Wide Pore 層析管柱 蛋白質體學分析 Biphasic 套件 Phosphopeptide 套件 pH Gradient 套件 YinYang 套件 高分子 Reversed Phase Ion Exchange 配件與耗材 Tubing Fitting       Small Molecule Separations Reversed Phase Columns: C18 Reversed phase HPLC is the most powerful and commonly used technique for the separation of pharmaceuticals. The mobile phases are usually a mixture of polar organic solvents and water. Separations can be isocratic or gradient and are based on analyte hydrophobicity - hydrophobic compounds are retained longer than polar compounds. A common issue with reversed phase separations is non-specific interactions between analytes and the column stationary phase. This undesired effect results from ionic interaction with un-reacted silanols or impurities in the silica gel. This non-specific interaction can cause peak tailing or adsorption as shown in Figures 2a and 2b. CTI’s AccuSil columns begin with exceptionally pure, highly characterized silica and use a proprietary bonding and end-capping techniques to eliminate free silanols. This results in columns with excellent peak shape and separation efficiency for all compounds including basic, neutral and acidic analytes. Superior Column Performance – Separation of Basic Compounds Many pharmaceuticals are basic, organic, nitrogen-containing compounds. During reversed phase chromatography, these nitrogen groups will react with non-reacted silanol groups on silica-based chromatography supports and can lead to chromatographic artifacts such as shifting retention times or the complete retention of a compound of interest. CTI’s reversed phase supports begin with the purest possible silica and are manufactured with a proprietary process that assures complete end-capping. Furthermore, each column is tested to make sure that no non-reacted silanols remains. The result is excellent chromatography as seen in the left panel below. AccuSil C18 Columns Particle Size (μm) Column Dimension (mm) Pore Size (A) Part Number 5 4.6 x 250 120 CTIODS546250 5 4.6 x 150 120 CTIODS546150 5 4.6 x 50 120 CTIODS546050 5 2.1 x 250 120 CTIODS521250 5 2.1 x 150 120 CTIODS521150 5 2.1 x 100 120 CTIODS521100 5 2.1 x 50 120 CTIODS521050 5 1.0 x 100 120 CTIODS510100 5 1.0 x 50 120 CTIODS510050 5 0.32 x 100 120 CTIODS5032100 5 2.1 x 100 120 CTIODS321100 5 2.1 x 50 120 CTIODS321050 5 1.0 x 100 120 CTIODS310100 5 1.0 x 50 120 CTIODS310050 5 0.32 x 100 120 CTIODS3032100 Applications     1. Simplified Multi-Dimensional Separations to Maximize Protein Detection in Poteomic Analyses     2. ASNS 53 poster-A Novel Method for Protein Identification with Integrated Multiple Dimensional Liquid Chromatography Coupled to Mass Spectrometry     3. Cholesterol- Lowering Drugs     4. Antihypertension Drugs     5. Arthritis Medicine     6. New! Phosphopeptide     7. New! Bi-phasic Kit     8. New! pH Gradient Kit     9. New! YinYang colimn Kit         網站佳解析度 1280*1024 本網站所所有圖文皆為科安所有 Copyright 2008 Kohan instruments Co., Ltd. All Rights Reserved.